A Handbook of Biochemistry
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Код:KS3146
Biochemistry courses provide students the knowledge and skills needed for future professional career in medicine and for research in the biotechnological and pharmaceutical industry
Second Edition
Biochemistry courses provide students the knowledge and skills needed for future professional career in medicine and for research in the biotechnological and pharmaceutical industry.
The purpose of A Handbook of Biochemistry. Review Study Questions and Laboratory Manuals - A Clinical Approach is to serve as a resource to enhance student learning of theories, techniques, and methodologies practiced during biochemistry teaching and in the research laboratory.
Content
Preface
Safety rules in the biochemistry laboratory
Seminar “Amino acids”: Proteinogenic amino acids
Seminar “Proteins І”: Structure and function of proteins
Seminar “Proteins ІІ”: Globular and fibrous proteins
Practical works: Proteins
Determination of the isoelectric point (pI) of casein
Determination of hemoglobin concentration by Drabkin’s reagent
Seminar “Enzymes I”: Enzymes as biocatalysts. Nomenclature and classification. izoenzymes. Water soluble vitamins as cofactors. Kinetics of enzyme action.
Seminar “Enzymes IІ”: Regulation of the enzyme activity. Reversible and irreversible inhibition of enzymes. Allosteric enzymes. Constitutive and inducible enzymes.
Practical works: Enzymes
Determination the absolute specificity of urease
Calculation of Km and Vmax by Lineweaver-Burke method
Seminar “Bioenergetics І”: Macroergic compounds. Biological oxidation. Oxidative phosphorilation. Structure of the respiratory chain
Seminar “Bioenergetics ІІ”: Short electron-transport chains. Free-radical oxidation and antioxidant defence
Practical works: Bioenergetics
Detection of cytochrome c oxidase and peroxidase in muscle tissue
Quantitative determination of total polyphenol content in plant extracts by Folin-Ciocalteu method
Seminar “Metabolism of carbohydrates І”: Digestion of carbohydrates. Transport of monosaccharides in cells. Glycolysis
Seminar “Metabolism of carbohydrates ІІ”: Degradation of pyruvate. Tricarboxylic acid cycle. Pentose phosphate Pathway
Seminar “Metabolism of carbohydrates ІII”: Gluconeogenesis. Glycogenesis. Glycogenolysis
Seminar “Metabolism of carbohydrates” ІV: Metabolism of mannose, fructose, galactose and lactose
Seminar “Metabolism of carbohydrates V”: Glycosaminoglycans. Glycoproteins
Seminar “Metabolism of carbohydrates VI”: Summary on carbohydrates metabolism
Practical works: Metabolism of carbohydrates
Alcoholic fermentation
Blood glucose curves of a healthy individual and a patient with type 1 diabetes
Determination of fructose in urine by Seliwanoff’s reagent
Seminar “Metabolism of lipids I”: Dietary lipiids digestion. Transport of exogenous lipids
Seminar “Metabolism of lipids II”: Lipolysis. Degradation of even and odd numbered fatty acids. Synthesis and use of ketone bodies
Seminar “Metabolism of lipids III”: Biosynthesis of fatty acids and triacylglycerols. Endogenous lipids in plasma
Seminar “Metabolism of lipids IV”: Biosynthesis of cholesterol, bile acids and bile salts. Cholesterol Transport in blood – role of HDL and LDL
Seminar “Metabolism of lipids V”: Complex lipids and eicosanoids
Practical works: Metabolism of lipids
Urine reagent strip test for ketone bodies
Quantative determination of triacylglycerols in serum
Quantative determination of total cholesterol in serum
Seminar “Metabolism of amino acids I”: Digestion of dietary proteins. Transamination of amino acids. Oxidative and nonoxidative deamination of amino acids
Practical work: Metabolism of amino acids
Quantitative determination of alanineaminotransferase (ALT) in serum
Seminar “Metabolism of porphyrins”: Heme biosynthesis and degradation. Porphyrias. Hyperbilirubinemias. Iron metabolism.
Practical work: Metabolism of porphyrins
Quantitative determination of total and direct bilirubin in serum
Seminar “Metabolism of nucleotides ІІ”: Biosynthesis and degradation of pyrimidine nucleotides. Biosynthesis of desoxyribonucleotides
Practical work: Metabolism of nucleotides
Determination of uric acid content in serum
Seminar “Nutrition and metabolism of xenobiotics”: Metabolism of xenobiotics and endobiotics. Nutrition
Seminar “Calcium metabolism”: hormones that regulate calcium and phosphate metabolism
Seminar “Intercellular signaling I”: General principles of intercellular signaling. Intracellular receptors. Receptors – ion channels
Seminar “Intercellular signaling II”: G-proteiN coupled receptors. With intrinsic enzimatic activity
Seminar “Molecular biology”: replication, transcription, translation. Cycle regulation. Oncogenes and tumor-suppressor genes. Regulation of gene expression
Recommended literature
